Valderrama, the host of the 1997 Ryder Cup, is among the new venues that will hold LIV Golf Series events in 2023.
Mayakoba’s El Camaleon in Mexico – a long-time PGA Tour venue – and regular European Tour host Sentosa in Singapore are the other new courses.
The Valderrama event is scheduled for the first weekend in July, when The Belfry will stage the British Masters.
“These venues have played host to signature moments in golf,” LIV Golf chief executive Greg Norman said.
“We’re excited to build new traditions for the sport while delivering a first-class fan experience at some of the world’s best courses.”
In July, LIV Golf announced it was expanding the series from eight tournaments to a 14-event league in 2023, with a prize fund of $405m (£336m).
El Camaleon, which is set to host the LIV tournament between 24 and 26 February, has staged a PGA Tour event since 2007.
LIV players Ian Poulter and Sergio Garcia are among the past winners of the Singapore Open on the European Tour at Sentosa, which will host a LIV tournament in April.
Valderrama, in Spain, had been a regular stop-off on the European Tour since 1988. It will host its first LIV event two weeks after the US Open and three weeks before The Open.
BBC golf correspondent Iain Carter
By adding Valderrama to its list of venues, LIV will be playing on continental Europe’s most famous golf course.
The Costa del Sol layout staged the 1997 Ryder Cup when Seve Ballesteros thrillingly led Europe to a narrow victory against a US team which contained a debuting Tiger Woods.
Valderrama staged the prestigious season-ending Tour Championship for two decades from 1988 and after that event shifted to Dubai, the Spanish course remained on the DP World Tour schedule hosting the Andalucia Masters.
While not a surprise, this is still a significant coup for the Saudi Arabian-funded LIV Tour, one that will further heighten tensions with the golfing establishment. The early July date means the tournament is being staged in the heart of the European season.
Whether it takes anything away from the British Masters, being played at the Belfry simultaneously, remains to be seen. LIV still need a television deal and streaming numbers for the initial eight tournaments staged this year were modest at best.